Sumaryam, - and Dwirini , Kartikasari (2013) ALGINATE MICROENCAPSULATION OF Vibrio sp FKC FOR GROUPER SEED VACCINE AGAINST VIBRIOSIS. In: Proseding Seminar Nasional : pengembangan luaran Penelitian Mendukung Dunia Industri, 10-11 Desember 2013, Surabaya.
Abstract
Humpback grouper ( Chromyleptes altivelis ) is a commercial fish of high economic value of fish production and mainstay of exports in Indonesia. Increased production of grouper needs to be accompanied by the beginning of the cultivation of improved seed production. There are obstacles that can hinder the development of cultivation, especially diseases caused by parasites, bacteria, viruses and fungi . Vibriosis is a disease caused by bacterial infection of the most attacking in the hatchery, is caused by bacteria of the genus Vibrio, among others Vibrio alginolyticus and Vibrio parahaemolyticus. Vibriosis in fish grouper resulted in a low survival rate ranged from 1.2 to 2.9 %, that can even lead to death of grouper seed to 100 % at the peak of the outbreak . It needs an effort of prevention to overcome these obstacles, because prevention will result in the use of antibiotic resistance in bacteria, the occurrence of residues in fish body and environment pollution. Countermeasures after fish infection will not be much help because of the quickly time of death. Prevention that can be done is by administering the vaccine . Vaccination is believed to provide a specific immunity against certain diseases in fish. Vaccinations can be given by injections, immersion or orally through feed. For fish with the seed size, oral vaccination is considered the most appropriate way. Constraints oral vaccination is antigen solubility in water before they could enter the fish body and damage antigen as it passes through the digestive tract due to the low pH, so the vaccine must be given a coating that protects it while the vaccine was in the water and prevent damage during passing through the digestive system of fish. Microencapsulation process is one way of protecting the antigen from the media and the solubility of the damage as it passes through the digestive tract of fish, so that the antigen can enter the fish body and will cause the immune response of the fish, which in turn can stimulate the formation of antibodies, characterized by increasing antibody titers. If the antibody has been formed, then the fish will be resistant when exposed to antigen, thus increasing the survival rate of the fish. This study aim is to coat the Vibrio sp Formaline Killed Cells with alginate microencapsulation process, so we can get the appropriate vaccine that suit for the grouper seed and provides effective protection against vibriosis, so as to improve the quality and quantity of grouper seed.
Actions (login required)
![[img]](http://eprints.upnjatim.ac.id/style/images/fileicons/application_pdf.png)
